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Catalog Number | orb782790 |
---|---|
Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Putative humanin peptide(MT-RNR2). |
Tested applications | ELISA |
Reactivity | Human |
Range | 31.25-2000 pg/mL |
Concentration | 2000 pg/mL |
Sensitivity | 12.3 pg/mL |
Alternative names | MT-RNR2 ELISA Kit, HN ELISA Kit, Humanin ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MT-RNR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MT-RNR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MT-RNR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MT-RNR2 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates |
Expiration Date | Please enquire. |
Human | |
28 pg/mL-1800 pg/mL | |
7 pg/mL |