Human Platelet-derived growth factor -BB (rHuPDGF-BB)

• Catalog Number: orb1495024
• Category: Proteins
• Description: Human Platelet-derived growth factor -BB Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: Approximately 12.4 KDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain).
• Protein Sequence: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
• Source: Escherichia coli.
• Biological Activity: Fully biologically active when compared to standard.The ED50 as determined by the dose-dependent stimulation of the proliferation of murine Balb/c 3T3 cells is ≤ 3.0ng/ml, corresponding to a specific activity of ≥ 3.3×105 units/mg.
• Endotoxins: Less than 1EU/μg of rHuPDGF-BB as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.