Human PCSK9 (D374Y) Protein

• Catalog Number: orb257745
• Category: Proteins
• Description: Human PCSK9 (D374Y), His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 13.8 kDa and 58.2 kDa
• Target: PCSK9
• UniProt ID: Q8NBP7
• Protein Sequence: AAI66619.1 (D374Y)
• Source: Human PCSK9 (D374Y), His Tag (orb257745) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Gln 31 - Gln 692
• Endotoxins: 1.0 EU per μg
• NCBI: D374, AAI66619.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.2 kDa respectively. The protein migrates as 17 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PCSK9 protein

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations