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Human Osteopontin Protein, His Tag
Catalog Number: orb257724
| Catalog Number | orb257724 |
|---|---|
| Category | Proteins |
| Description | Human Osteopontin, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
| Reactivity | Human |
| Tag | C-6×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 33.0 kDa |
| Target | Osteopontin, OPN |
| UniProt ID | P10451 |
| Source | Human Osteopontin, His Tag (orb257724) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Ile 17 - Asn 300 |
| Endotoxins | 0.1 EU per μg |
| NCBI | AAH07016 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | SPP1,BNSP,OPN,Uropontin,Nephropontin,Osteopontin,B Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Osteopontin protein
Osteopontin Protein, Human, Recombinant (aa 17-300, His) [orb1960226]
98.00%
34.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mgOsteopontin Protein, Human, Recombinant (aa 17-166, His) [orb1953021]
95.30%
The recombinant human SPP1 consists of 185 amino acids and predicts a molecular mass of 20.64 kDa.
100 μgOsteopontin Protein, Human, Recombinant (His) [orb1958343]
91.60%
35 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation)
1 mg, 100 μgRecombinant Human Osteopontin Protein, His Tag [orb1551076]
> 87% by SDS-PAGE.
Recombinant Human Osteopontin Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ile17-Asn300) of human Osteopontin (Accession #NP_001035147.1) fused with a 6��His Tag at the C-terminus.
50 μg, 100 μg
