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Catalog Number | orb257724 |
---|---|
Category | Proteins |
Description | Human Osteopontin, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 80% |
Conjugation | Unconjugated |
MW | 33.0 kDa |
Target | Osteopontin |
UniProt ID | P10451 |
Source | Human Osteopontin, His Tag (orb257724) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # AAH07016). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Ile 17 - Asn 300 |
Endotoxins | 0.1 EU per μg |
NCBI | AAH07016 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | SPP1,BNSP,OPN,Uropontin,Nephropontin,Osteopontin,B Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Osteopontin protein
Human | |
0.78 ng/mL-50 ng/mL | |
0.195 ng/mL |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
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