Human Oncostatin-M protein

• Catalog Number: orb80095
• Category: Proteins
• Description: Oncostatin-M Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids and having a molecular mass of 26kDa. The OSM is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: OSM
• Solubility (25°C): It is recommended to reconstitute the lyophilized Oncostatin in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Ile-Gly.
• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is 2 ng/ml, corresponding to Specific Activity of 500,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from concentrated (1mg/ml) solution containing no additives.
• Alternative names: OSM protein, MGC20461 protein
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.45 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Oncostatin as Reference.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Oncostatin-M protein