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Catalog Number | orb257725 |
---|---|
Category | Proteins |
Description | Human Oncostatin M Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Arg 252 (Accession # NP_065391.1). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 25.8 kDa |
Target | Oncostatin M |
UniProt ID | P13725 |
Source | Human Oncostatin M Protein, premium grade (orb257725) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Arg 252 (Accession # NP_065391.1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human Oncostatin M Protein, premium grade at 5 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag with a linear range of 0.01-0.312 μg/mL (QC tested). |
Expression Region | Ala 26 - Arg 252 |
Endotoxins | 0.1 EU per μg |
NCBI | NP_065391.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | OSM,MGC20461,Oncostatin M Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Oncostatin M protein
Immobilized Human Oncostatin M Protein, premium grade (Cat. No. orb257725) at 5 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 0.01-0.312 μg/mL (QC tested).
Unconjugated | |
95% | |
83.0 kDa | |
Human OSMR, His Tag (orb1496186) is expressed from human 293 cells (HEK293). It contains AA Glu 28 - Met 740 (Accession # Q99650-1). |
Unconjugated | |
90% | |
22.2 kDa | |
Human Oncostatin M (OSM) (26-221), Tag Free (orb1496309) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Arg 221 (Accession # P13725). |
Greater than 85% as determined by SDS-PAGE. | |
24.0 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
38.2 kDa | |
E.coli |
> 95% as determined by SDS-PAGE | |
35 kDa |