Human Nuclear Antigen Antibody

• Catalog Number: orb248661
• Category: Antibodies
• Description: This human nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 235-1
• Tested applications: FACS, ICC, IF, IHC-Fr, IP
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunocytochemistry (acetone-fixed cells): 1-2ug/ml for 30 min at RT,Immunohistochemistry (Frozen): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Nuclear Antigen antibody is available for research use only.
• Storage: Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear Antigen antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

ICC staining of paraformaldehyde-fixed human HeLa cells with Nuclear Antigen antibody (red, clone 235-1) and counterstained with DyLight 488 conjugated Phalloidin (green).

IHC testing of acetone-fixed frozen human tonsil tissue with Nuclear Antigen antibody (clone 235-1).

FACS testing of MCF-7 cells: Black=no Ab; Green=isotype control; Red=Nuclear Antigen antibody PE conjugate

FACS testing of HeLa cells: Black=no Ab; Green=isotype control; Red=Nuclear Antigen antibody PE conjugate

Flow cytometry testing of PFA-fixed human MCF7 cells with Human Nuclear Antigen antibody (clone 235-1); Red=isotype control, Blue=Human Nuclear Antigen antibody.

Flow cytometry testing of PFA-fixed human HeLa cells with Human Nuclear Antigen antibody (clone 235-1); Red=isotype control, Blue=Human Nuclear Antigen antibody.

Flow cytometry testing of PFA-fixed human Jurkat cells with Human Nuclear Antigen antibody (clone 235-1); Red=isotype control, Blue=Human Nuclear Antigen antibody.

Flow cytometry testing of PFA-fixed human K562 cells with Human Nuclear Antigen antibody (clone 235-1); Red=isotype control, Blue=Human Nuclear Antigen antibody.

ICC staining of paraformaldehyde-fixed human K562 cells with Nuclear Antigen antibody (green, clone 235-1) and counterstained with Phalloidin (red).

ICC staining of paraformaldehyde-fixed human MCF7 cells with Nuclear Antigen antibody (green, clone 235-1) and counterstained with Phalloidin (red).

Flow cytometry testing of PFA-fixed human Raji cells with Human Nuclear Antigen antibody (clone 235-1); Red=isotype control, Blue=Human Nuclear Antigen antibody.

SDS-PAGE analysis of purified, BSA-free Nuclear Antigen antibody (clone 235-1) as confirmation of integrity and purity.