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Human NT5E protein
Catalog Number: orb594861
| Catalog Number | orb594861 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human 5'-nucleotidase(NT5E),partial (Active) |
| Tag | C-terminal 6xHis-tagged |
| Form/Appearance | Liquid |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| MW | 58.76 kDa |
| UniProt ID | P21589 |
| Protein Sequence | WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK |
| Protein Length | Partial |
| Source | Mammalian cell |
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Specific activity as determined by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP) is greater than 15000 pmol/min/µg. The orthophosphate product is measured by a malachite green phosphate detection kit. |
| Expression Region | 27-547aa |
| Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Buffer/Preservatives | 0.2 μm filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5 |
| Alternative names | 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73 Read more... |
| Note | For research use only |
| Application notes | Partial |
| Expiration Date | 6 months from date of receipt. |
Human NT5E protein [orb392209]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Human cells
50 μg, 500 μg, 10 μgHuman NT5E protein [orb704591]
Greater than 90% as determined by SDS-PAGE.
84.6 kDa
E.coli
20 μg, 100 μg, 1 mgRecombinant Human CD73/NT5E Protein, His Tag [orb1553383]
≥90% as determined by SDS-PAGE
This protein contains the human NT5E(Met1-Ser549) was fused with the C-terminal His Tag and expressed in Mammalian cells.
50 μg, 100 μg
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