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Human Neuropeptide Y (NPY) ELISA Kit

Catalog Number: orb776311

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb776311
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y(NPY).
Tested applicationsELISA
ReactivityHuman
Range31.25-2000 pg/mL
Concentration2000 pg/mL
UniProt IDP01303
Sensitivity9.35 pg/mL
Alternative namesNP-Y ELISA Kit, PYY4 ELISA Kit, CPON ELISA Kit, Pr
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NoteFor research use only
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPY. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPY, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPY in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
Human Neuropeptide Y (NPY) ELISA Kit

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