Human Neprilysin / MME / CD10 Protein

• Catalog Number: orb257693
• Category: Proteins
• Description: Human Neprilysin, Tag Free is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 80.0 kDa
• Target: Neprilysin
• UniProt ID: P08473
• Protein Sequence: NP_000893.2
• Source: Human Neprilysin, Tag Free (orb257693) is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2).
• Expression System: HEK293
• Biological Origin: Human
• Expression Region: Tyr 52 - Trp 750
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000893.2
• Storage: -20°C
• Buffer/Preservatives: 20 mM Tris, 100 mM NaCl, pH8.0
• Alternative names: MME,CALLA,CD10,DKFZp686O16152,MGC126681,MGC126707,
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 80.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Willard, Joshua R. et al. Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels Diabetologia, 60, 701-708 (2017)

PubMed: 27933334

SDS-PAGE analysis of Human Neprilysin protein