Human NE(Noradrenaline) ELISA Kit

• Catalog Number: orb1088246
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Noradrenaline(NE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Noradrenaline(NE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Noradrenaline(NE) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 78.13-5000 pg/mL
• Concentration: 5000 pg/mL
• Sensitivity: 24.1 pg/mL
• Protein Length: 2h
• Alternative names: NA, NAd, Norepinephrine, Norepi
• Note: For research use only
• Application notes: standard: 5000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NE in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.