Human MOTS-c ELISA Kit

• Catalog Number: orb781469
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mitochondrial Open Reading Frame Of The 12S rRNA-c(MOTS-c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c(MOTS-c).
• Tested applications: ELISA
• Reactivity: Human
• Range: 3.13-200 ng/mL
• Concentration: 200 ng/mL
• UniProt ID: A0A0C5B5G6
• Sensitivity: 1.01 ng/mL
• Protein Length: 2h
• Alternative names: MOTS-c
• Note: For research use only
• Application notes: standard: 200 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.