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Catalog Number | orb257689 |
---|---|
Category | Proteins |
Description | Human MICA, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1). |
Reactivity | Human |
Tag | C-7×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 33.8 kDa |
Target | MICA |
UniProt ID | Q29983 |
Protein Sequence | AAH16929.1 |
Source | Human MICA, His Tag (orb257689) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Glu 24 - Gln 308 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH16929.1 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | MIC-A Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.8 kDa. The protein migrates as 45-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human MICA protein
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 12.5 kDa after removal of the signal peptide. | |
Mammalian |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 37.9 kDa after removal of the signal peptide. The apparent molecular mass of MICAa3(203-306)-mFc is approximately 35-70 kDa due to glycosylation. | |
Mammalian |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Human | |
1.56 ng/mL-100 ng/mL | |
0.39 ng/mL |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
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