Human MGMT protein

• Catalog Number: orb392136
• Category: Proteins
• Description: Recombinant human MGMT protein is produced by our E. coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM EDTA, pH 8.0.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Target: MGMT
• Entrez: 4255
• UniProt ID: P16455
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
• Source: E. coli
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM EDTA, pH 8.0.
• Alternative names: Methylated-DNA--protein-cysteine methyltransferase
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.