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Catalog Number | orb707002 |
---|---|
Category | Proteins |
Description | Recombinant Human Mesothelin Protein is produced by mammalian expression system and the target gene encoding Glu296-Ser598 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | MSLN |
Entrez | 10232 |
UniProt ID | Q13421 |
Protein Sequence | EVEKTACPSG KKAPEIDESL IFYKKWELEA CVDAALLATQ MDRVNAIPFT YEQLDVLKHK LDELYPQGYP ESVIQHLGYL FLKMSPEDIR KWNVTSLETL KALLEVNKGH EMSPQVATLI DRFVKGRGQL DKDTLDTLTA FYPGYLCSLS PEELSSVPPS SIWAVRPQDL DTCDPRQLDV LYPKARLAFQ NMNGSEYFVK IQSFLGGAPT EDLKALSQQN VSMDLATFMK LRTDAVLPLT VAEVQKLLGP HVEGLKAEER HRPVRDWILR QRQDDLDTLG LGLQGGIPNG YLVLDLSMQE ALSDIEGRMD EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | MPF protein, Mesothelin protein, CAK1 antigen prot Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of Mesothelin(37-286)-His is approximately 25-35 kDa due to glycosylation. | |
Mammalian |
Human | |
0.312 pmol/ml-20 pmol/ml | |
0.078 pmol/ml |
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