Human Mesothelin (296-580) Protein, mFc-His Tag

• Catalog Number: orb689392
• Category: Proteins
• Description: Recombinant human Mesothelin(296-580) protein with C-terminal mouse Fc and 6×His tag
• Reactivity: Human
• Tag: C-Mouse Fc and 6×His Tag
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• MW: The protein has a predicted molecular mass of 59.3 kDa after removal of the signal peptide.
• Target: Mesothelin
• UniProt ID: Q13421
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Alternative names: MSLN;Mesothelin;MPF
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human PD-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human PD1, mFc-His tagged protein (orb689386) can bind Anti-PD-1 Neutralizing antibody orb689311 in a linear range of 0.24-6.49 ng/ml.

Flow cytometry analysis with 2 μg/ml Human PD-1 Protein, mFc-His tag (orb689386) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2 μg/mL Human PD-1 Protein, mFc-His tag (orb689386) followed by PE-conjugated Goat anti-mouse IgG secondary antibody.