Human MCP2 protein

• Catalog Number: orb80141
• Category: Proteins
• Description: Monocyte Chemotactic Protein-2 Human Recombinant produced in E.coli is non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The MCP2 is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: MCP 2
• Solubility (25°C): It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.
• Biological Origin: E.coli
• Biological Activity: The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein was lyophilized from concentrated (1mg/ml) sterile solution containing no additives.
• Alternative names: Small inducible cytokine A8 protein, CCL8 protein,
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human MCP2 protein