Human Macrophage Colony Stimulating Factor (rHuM-CSF)

• Catalog Number: orb1495033
• Category: Proteins
• Description: M-CSF, also known as CSF-1, is a four α helical bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: Approximately 42.0 kDa, a covalently linked homodimer, of two 21.0 kDa polypeptide monomers (1-149 amino acid of human M-CSF).
• Protein Sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDKACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ
• Source: Baculovirus infected Silkworm
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine M-NSF-60 cells is less than 3.0 ng/ml, corresponding to a Specific Activity of ＞3.3 x 105 IU/mg.
• Endotoxins: Less than 1EU/mg of rHuM-CSF as determined by LAL method.
• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2mm filtered solution in 20mM PB, containing 1% HSA and 3% Mannitol.
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Expiration Date: 6 months from date of receipt.