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Catalog Number | orb257371 |
---|---|
Category | Proteins |
Description | Human M-CSF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 96% |
Conjugation | Unconjugated |
MW | 55.3 kDa |
Target | M-CSF R |
UniProt ID | P07333 |
Source | Human M-CSF R, His Tag (orb257371) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Biotinylated Human M-CSF, His, immobilized on SA Chip can bind Human M-CSF R, His Tag with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested). |
Expression Region | Ile 20 - Glu 512 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_005202.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human M-CSF R protein
Biotinylated Human M-CSF, His, Avitag immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. orb257371) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Unconjugated | |
95% | |
80.6 kDa | |
Human M-CSF R Protein, Fc Tag (orb257372) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2). |