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Catalog Number | orb257990 |
---|---|
Category | Proteins |
Description | Human LIF Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 19.9 kDa |
Target | LIF |
UniProt ID | P15018 |
Source | Human LIF Protein, premium grade (orb257990) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human LIF Protein, premium grade at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag with a linear range of 4-63 ng/mL (QC tested). |
Expression Region | Ser 23 - Phe 202 |
Endotoxins | 0.1 EU per μg |
NCBI | AAH69540.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | LIF,CDF,DIA,HILDA,MLPLI Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human LIF protein
Immobilized Human LIF Protein, premium grade (Cat. No. orb257990) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 4-63 ng/mL (QC tested).
Greater than 95% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |
> 98% as determined by SDS-PAGE and HPLC. | |
19.7 kDa | |
E.Coli |
Unconjugated | |
95% | |
115.5 kDa | |
Human LIF R, Fc Tag (orb257674) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - Ser 833 (Accession # P42702-1). |