Human Latent TGF-beta 1 Protein, His Tag

Catalog Number: orb383501

Catalog Number	orb383501
Category	Proteins
Description	Human Latent TGFB1, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (Accession # P01137-1).
Reactivity	Human
Tag	N-10×His
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	42.6 kDa (monomer)
Target	Latent TGF-beta 1, TGFB1
UniProt ID	P01137
Protein Sequence	NP_000651.3
Source	Human Latent TGFB1, His Tag (orb383501) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (Accession # P01137-1).
Expression System	HEK293
Biological Origin	Human
Expression Region	Leu 30 - Ser 390
Endotoxins	1.0 EU per μg
NCBI	NP_000651.3
Storage	-20℃
Buffer/Preservatives	PBS, pH7.4
Alternative names	Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP, Read more...
Note	For research use only
Application notes	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 42.6 kDa (monomer). The protein migrates as 38-43kDa and 50 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation and Interchain disulfide bond.
Expiration Date	6 months from date of receipt.

SDS-PAGE analysis of Human TGF-beta 1 protein

Purity:	98.00%
MW:	108.72 kDa (predicted); (72.33, 40.89 and 13.99) kDa (reducing conditions)

Purity:	98.00%
MW:	45.9 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 43-48 kDa and 50-60 kDa based on Tris-Bis PAGE result.

Purity:	99.50%
MW:	42.4 kDa (predicted); 16, 38 and 55 kDa (reducing condition, due to glycosylation); 110 kDa (non-reduced condition, due to glycosylation)