Human Interleukin 23 (IL23) ELISA Kit

• Catalog Number: orb775434
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 23(IL23).
• Tested applications: ELISA
• Reactivity: Human
• Range: 7.82-500 pg/mL
• Concentration: 500 pg/mL
• Sensitivity: 2.7 pg/mL
• Alternative names: IL23A ELISA Kit, P19 ELISA Kit, IL23P19 ELISA Kit,
• Note: For research use only
• Application notes: standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL23. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL23. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL23 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.