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Catalog Number | orb753279 |
---|---|
Category | Proteins |
Description | Recombinant human Insulin protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | No tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 5.8 KDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4 |
UniProt ID | P01308 |
Source | E.Coli |
Expression System | chain a+chain b |
Expression Region | chain a+chain b |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4 |
Alternative names | IDDM protein, IDDM1 protein, IDDM2 protein, ILPR p Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
> 97 % by SDS-PAGE and HPLC analyses. | |
The apparent molecular weight is about 30 kDa in SDS-PAGE under reducing conditions, containing 237 amino acids. | |
Insect Cell |
> 98 % by SDS-PAGE and HPLC analyses. | |
Approximately 28.8 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids. | |
Escherichia coli |
Unconjugated | |
95% | |
129.3 kDa | |
Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
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