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Catalog Number | orb752951 |
---|---|
Category | Proteins |
Description | IL6 Human Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 189 amino acids (30-212aa) and having a molecular mass of 21.6kDa.(Migrates at 18-28kDa on SDS-PAGE under reducing conditions).IL6 is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENNLNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKVLIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQMHHHHHH |
Source | Sf9, Baculovirus cells |
Biological Activity | The ED50, determined by cell proliferation assay using TF-1 human erythroleukemic cell, is 0.2 - 0.8 ng/ml . |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | IL6 protein (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
Alternative names | BSF2, HGF, HSF, IFNB2, IL-6. Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
> 95% as determined by SDS-PAGE | |
20.8 kDa | |
Human IL-6, premium grade (orb257601) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000592.3). |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
20.9 kDa | |
E.coli |
Unconjugated | |
90% | |
23.6 kDa | |
Mouse IL-6, His Tag (orb750344) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1). |
Unconjugated | |
95% | |
22.9 kDa | |
Cynomolgus IL-6, His Tag (orb1152434) is expressed from human 293 cells (HEK293). It contains AA Ala 28 - Met 212 (Accession # P79341-1). |