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Catalog Number | orb594784 |
---|---|
Category | Proteins |
Description | Recombinant Human Interleukin-6(IL6),partial (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 20.9 kDa |
UniProt ID | P05231 |
Protein Sequence | VPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Biological Activity | The ED50 as determined in a cell proliferation assay using TF‑1 human erythroleukemic cells is 20-100 pg/ml. |
Expression Region | 30-212aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM HAc-NaAc, 150 mM NaCl, pH 5.0 |
Alternative names | Interleukin-6; IL-6; B-Cell Stimulatory Factor 2; Read more... |
Note | For research use only |
Application notes | Partial |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Unconjugated | |
> 95% as determined by SDS-PAGE | |
20.8 kDa | |
Human IL-6, premium grade (orb257601) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000592.3). |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 47.9 kDa after removal of the signal peptide. | |
Mammalian |
Unconjugated | |
90% | |
23.6 kDa | |
Mouse IL-6, His Tag (orb750344) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1). |
Unconjugated | |
95% | |
22.9 kDa | |
Cynomolgus IL-6, His Tag (orb1152434) is expressed from human 293 cells (HEK293). It contains AA Ala 28 - Met 212 (Accession # P79341-1). |