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Catalog Number | orb594783 |
---|---|
Category | Proteins |
Description | Recombinant Human Interleukin-5(IL5) (Active) |
Tag | C-terminal 6xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 14.2 kDa |
UniProt ID | P05113 |
Protein Sequence | IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES |
Protein Length | Full Length of Mature Protein |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | The ED50 as determined in a cell proliferation assay using TF‑1 human erythroleukemic cells is 0.1-0.5 ng/ml. |
Expression Region | 20-134aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4 |
Alternative names | Interleukin-5;IL-5;B-cell differentiation factor I Read more... |
Note | For research use only |
Application notes | Full Length of Mature Protein |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
> 98% as determined by SDS-PAGE and HPLC. | |
13.3 kDa | |
E.Coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
15.0 kDa | |
Human IL-5, His Tag, premium grade (orb612148) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1). |
Unconjugated | |
90% | |
15.2 kDa | |
Rabbit IL-5, His Tag (orb750340) is expressed from human 293 cells (HEK293). It contains AA Met 20 - Ser 134 (Accession # G1SL79-1). |
Unconjugated | |
90% | |
15.0 KDa | |
Mouse IL-5, His Tag (orb762422) is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1). |