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Catalog Number | orb594802 |
---|---|
Category | Proteins |
Description | Recombinant Human Interleukin-4(IL4) (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 14.97 kDa |
UniProt ID | P05112 |
Protein Sequence | HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS |
Protein Length | Full Length of Mature Protein |
Source | Mammalian cell |
Biological Origin | Homo sapiens (Human) |
Biological Activity | The ED50 as determined in a cell proliferation assay using TF‑1 human erythroleukemic cells is 0.01-0.05 ng/ml. |
Expression Region | 25-153aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4 |
Alternative names | Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; Read more... |
Note | For research use only |
Application notes | Full Length of Mature Protein |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Greater than 95% as determined by SDS-PAGE. | |
15.1 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
16 kDa | |
Mammalian cell |
> 98% as determined by SDS-PAGE and HPLC. | |
15.0 kDa | |
E.Coli |
Unconjugated | |
90% | |
15.0 kDa | |
Human IL-4, premium grade (orb257598) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1). |
Unconjugated | |
90% | |
41.4 kDa | |
Cynomolgus IL-4, Fc Tag (orb1152433) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P79339-1). |