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Catalog Number | orb391967 |
---|---|
Category | Proteins |
Description | Recombinant human IL25 protein is produced by our mammalian expression system and the target gene encoding Tyr33-Gly177 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL25 |
Entrez | 64806 |
UniProt ID | Q9H293 |
Protein Sequence | YSHWPSCCPS KGQDTSEELL RWSTVPVPPL EPARPNRHPE SCRASEDGPL NSRAISPWRY ELDRDLNRLP QDLYHARCLC PHCVSLQTGS HMDPRGNSEL LYHNQTVFYR RPCHGEKGTH KGYCLERRLY RVSLACVCVR PRVMGVDHHH HHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 1mM EDTA, pH 8.0. |
Alternative names | IL17E; Interleukin-25; IL-25; Interleukin-17E; IL- Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.9 kDa after removal of the signal peptide. The apparent molecular mass of IL25-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Human | |
0.156 ng/mL-10 ng/mL | |
0.039 ng/mL |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
SDS-PAGE: 100%; SEC-HPLC: 97% | |
20-26 KDa (reducing condition) |