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Catalog Number | orb391961 |
---|---|
Category | Proteins |
Description | Recombinant human IL21R protein is produced by our mammalian expression system and the target gene encoding Cys20-Pro236 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL21R |
Entrez | 50615 |
UniProt ID | Q9HBE5 |
Protein Sequence | CPDLVCYTDY LQTVICILEM WNLHPSTLTL TWQDQYEELK DEATSCSLHR SAHNATHATY TCHMDVFHFM ADDIFSVNIT DQSGNYSQEC GSFLLAESIK PAPPFNVTVT FSGQYNISWR SDYEDPAFYM LKGKLQYELQ YRNRGDPWAV SPRRKLISVD SRSVSLLPLE FRKDSSYELQ VRAGPMPGSS YQGTWSEWSD PVIFQTQSEE LKEGWNPVDH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | NILR; Interleukin-21 receptor; IL-21 receptor; IL- Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
26.9 kDa | |
Human IL-21 R, His Tag (orb257627) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9HBE5-1). |
Unconjugated | |
95% | |
51.5 kDa | |
Human IL-21 R, Fc Tag (orb257628) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (Accession # Q9HBE5-1). |
Unconjugated | |
95% | |
51.0 kDa | |
Cynomolgus IL-21 R, Fc Tag (orb1496201) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Glu 232 (Accession # XP_005591578.2). |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.7 kDa after removal of the signal peptide.The apparent molecular mass of IL21R-hFc is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 25.4 kDa after removal of the signal peptide.The apparent molecular mass of IL21R-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |