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Catalog Number | orb391959 |
---|---|
Category | Proteins |
Description | Recombinant human IL21 protein is produced by our E. coli expression system and the target gene encoding Gln23-Ser155 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL21 |
Entrez | 59067 |
UniProt ID | Q9HBE4 |
Protein Sequence | MQGQDRHMIR MRQLIDIVDQ LKNYVNDLVP EFLPAPEDVE TNCEWSAFSC FQKAQLKSAN TGNNERIINV SIKKLKRKPP STNAGRRQKH RLTCPSCDSY EKKPPKEFLE RFKSLLQKMI HQHLSSRTHG SEDS |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Interleukin-21; IL-21; Za11 Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
15.4 kDa | |
E.Coli |
Unconjugated | |
95% | |
41.9 kDa | |
Human IL-21, Fc Tag (orb545751) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # Q9HBE4-1). |
Unconjugated | |
95% | |
17.4 kDa | |
Cynomolgus IL-21, His Tag (orb1496202) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # XP_005555921.1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 16.3 kDa after removal of the signal peptide.The apparent molecular mass of IL21-His is approximately 15-25 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.6 kDa after removal of the signal peptide.The apparent molecular mass of IL21-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |