Human IL17A protein

• Catalog Number: orb80077
• Category: Proteins
• Description: IL17A Human Recombinant produced in E.coli is homodimeric, non-glycosylated polypeptide chain containing total of 264 amino acids (2 chains of 132 aa) and having a molecular mass of 30,250 Dalton. The IL-17 is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: IL17
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interleukin 17 in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.
• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2ng/ml.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Sterile filtered lyophilized (freeze-dried) powder, with 20 mM Citrate, 0.1M NaCl, pH4.5.
• Alternative names: CTLA-8 protein, IL-17 protein, IL-17A protein, Cyt
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-17 as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL17A protein