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Catalog Number | orb2701965 |
---|---|
Category | Proteins |
Description | Human IL12A Protein, Fc Tag |
Reactivity | Human |
Purity | > 95%, determined by SDS-PAGE |
Target | IL12A |
Source | This protein contains the p35 subunit of human IL12, termed as IL12A (P29459) (Met 1-Ser 219) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |
Expression System | HEK293 Cells |
Endotoxins | > 95%, determined by SDS-PAGE |
Alternative names | IL12A, interleukin 12A, P35, CLMF, NFSK, NKSF1, IL Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
49.2 kDa | |
Human IL-12A, Fc Tag (orb257707) is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
> (59.8+33.3)%, determined by SDS-PAGE | |
This protein contains the p35 subunit of human IL12, termed as IL12A (NP_0008732) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B, (NP_0057462) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2 . The two plasmids were co-expressed and the heterodimer was purified and expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; This protein contains the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
> 95% by SDS-PAGE. | |
Recombinant Human IL12A/NKSF1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg23-Ser219) of human IL12A/NKSF1 (Accession #NP_002178.2) fused with an Fc, 6��His Tag at the C-terminus. |