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Catalog Number | orb80070 |
---|---|
Category | Proteins |
Description | IL11 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19256.29 Dalton. The IL-11 is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | IL11 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 11 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Pro-Pro-Gly.N-terminal methionine has been completely removed enzymatically. |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of murine 7TD1 was found to be 10ng/ml, corresponding to Specific Activity of 100,000 IU/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) solution with no additives. |
Alternative names | AGIF protein, Adipogenesis inhibitory factor prote Read more... |
Note | For research use only |
Application notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.95 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-11 as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
19.1 kDa | |
E.Coli |
Unconjugated | |
90% | |
21.3 kDa | |
Cynomolgus IL-11 Protein, His Tag (orb1496237) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20808). |
Unconjugated | |
95% | |
45.6 kDa | |
Mouse IL-11 Protein, Fc Tag (orb1496246) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P47873). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of IL11-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. | |
P. pastoris |
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