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Catalog Number | orb1184783 |
---|---|
Category | Proteins |
Description | Human IL-9 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Ile 144 (Accession # P15248-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 16.0 kDa |
Target | IL-9 |
UniProt ID | P15248 |
Source | Human IL-9, His Tag (orb1184783) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Ile 144 (Accession # P15248-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-9 Protein, His Tag, premium grade at 5 μg/mL (100 μL/well) can bind Human IL-9R, Fc Tag with a linear range of 5-78 ng/mL (QC tested). |
Expression Region | Gln 19 - Ile 144 |
Endotoxins | 0.1 EU per μg |
NCBI | NP_000581.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Interleukin-9,IL-9,Cytokine P40 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.0 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IL-9 Protein, His Tag, premium grade (Cat. No. orb1184783) at 5 μg/mL (100 μL/well) can bind Human IL-9R, Fc Tag with a linear range of 5-78 ng/mL (QC tested).
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 14.9 kDa after removal of the signal peptide. The apparent molecular mass of IL9-His is approximately 25-55 kDa due to glycosylation. | |
Mammalian |
Greater than 85.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
> 97%, determined by SDS-PAGE | |
This protein contains the human IL9 (NP_0005811) (Gln 19-Il4 144) was expressed and purified, fused with a polyhistidine tag at the C-terminus and expressed from Baculovirus-Insect Cells. |
> 97 % as determined by SDS-PAGE | |
15.5 kDa (predicted); 19-26 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Human IL-9 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Glu19-Ile144) of human IL-9 (Accession #P15248) fused with a 6��His Tag at the C-terminus. |