Human IL-7 R alpha / CD127 Protein

• Catalog Number: orb402998
• Category: Proteins
• Description: Human IL-7 R alpha, Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1 (I66T,V138I)).
• Reactivity: Human
• Tag: C-mFc(mIgG2a)
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 52.0 kDa
• Target: IL-7 R alpha
• UniProt ID: P16871
• Source: Human IL-7 R alpha, Mouse IgG2a Fc Tag (orb402998) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1 (I66T,V138I)).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag on CM5 chip via anti-mouse antibodies surface can bind Human IL-7, Tag Free with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Glu 21 - Gly 236
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL7Ra,CD127
• Note: For research use only
• Application notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. orb402998) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. orb257603) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).