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Catalog Number | orb257601 |
---|---|
Category | Proteins |
Description | Human IL-6, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591.1). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | > 95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
MW | 20.8 kDa |
Target | IL-6 |
UniProt ID | P05231 |
Source | Human IL-6, premium grade (orb257601) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000592.3). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Val 30 - Met 212 |
Endotoxins | 0.01 EU per μg |
NCBI | NP_000591 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant |
Alternative names | IL6,Interleukin-6,BSF2,HSF,IFNB2 Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL6 protein
Human IL-6, stimulates proliferation of TF-1 human erythroleukemic cell line. The specific activity of Human IL-6 is > 1.00×10^8 IU/mg, which is calibrated against human IL-6 WHO International Standard
The Cell based assay shows that Human IL-6 Protein is stable at 37°C for 48 hours
Unconjugated | |
95% | |
39.4 kDa | |
Human IL-6 R alpha, His Tag (orb257625) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1). |
Greater than 95% as determined by SDS-PAGE. | |
20.9 kDa | |
E.coli |
> 96% as determined by SDS-PAGE and HPLC. | |
20.7 kDa | |
E.Coli |