Human IL-6 Mouse Monoclonal Antibody

• Catalog Number: orb499747
• Category: Antibodies
• Description: Human IL-6 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1C4
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human IL-6 protein (30-212/212aa (C-6x His-Tag)aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: IL6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Interleukin BSF 2; B cell differentiation factor;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HUVEC (H) cells were treated with or without LPS (0.5 µg/ml, 24h) and Brefeldin A (300 ng/ml, 20h), 25 µg total protein per lane of cell lysates probed with human IL-6 monoclonal antibody, unconjugated (orb499747) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Sample: Lane 1: Recombinant human IL-6 protein, C-His, Lane 2: Recombinant human IL-6 protein, C-His, Primary: Anti-human IL-6 (orb499747) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 15 kDa.