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Human IL-5 R alpha Protein, His Tag

Catalog Number: orb257612

DispatchUsually dispatched within 5-10 working days
$ 640.00
Catalog Numberorb257612
CategoryProteins
DescriptionHuman IL-5 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555).
ReactivityHuman
TagC-10×His
Form/AppearancePowder
Purity85%
ConjugationUnconjugated
MW37.7 kDa
TargetIL-5 R alpha, CD125
UniProt IDQ01344
SourceHuman IL-5 R alpha, His Tag (orb257612) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555).
Expression SystemHEK293
Biological OriginHuman
Biological ActivityLoaded Biotinylated Human IL-5, His, on SA Biosensor, can bind Human IL-5 R alpha, His Tag with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Expression RegionAsp 21 - Glu 335
Endotoxins1.0 EU per μg
NCBINP_000555
Storage-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Buffer/PreservativesPBS, pH7.4
Alternative namesIL5Ra,CD125
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NoteFor research use only
Application notesThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Human IL-5 R alpha Protein, His Tag

SDS-PAGE analysis of Human IL-5 R alpha protein

Human IL-5 R alpha Protein, His Tag

Loaded Biotinylated Human IL-5, His, Avitag on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. orb257612) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Human IL5RA Protein, His Tag [orb689433]

    The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg
  • IL-5R alpha/CD125 Protein, Human, Recombinant (His & Avi), Biotinylated [orb1960616]

    98.00%

    38.7 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

    1 mg, 500 μg, 100 μg
  • IL-5R alpha/CD125 Protein, Human, Recombinant (His & Avi) [orb1960617]

    98.00%

    38.7 kDa (predicted). Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.

    1 mg, 500 μg, 100 μg
  • Recombinant Human IL-5 R alpha/CD125 Protein, His Tag [orb1550877]

    > 95% by SDS-PAGE.

    Recombinant Human IL-5 R alpha/CD125 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Asp21-Glu335) of human IL-5 R alpha/CD125 (Accession #Q01344) fused with a 6��His Tag at the C-terminus.

    50 μg