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Catalog Number | orb257612 |
---|---|
Category | Proteins |
Description | Human IL-5 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 85% |
Conjugation | Unconjugated |
MW | 37.7 kDa |
Target | IL-5 R alpha, CD125 |
UniProt ID | Q01344 |
Source | Human IL-5 R alpha, His Tag (orb257612) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Biotinylated Human IL-5, His, on SA Biosensor, can bind Human IL-5 R alpha, His Tag with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
Expression Region | Asp 21 - Glu 335 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000555 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL5Ra,CD125 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-5 R alpha protein
Loaded Biotinylated Human IL-5, His, Avitag on SA Biosensor, can bind Human IL-5 R alpha, His Tag (Cat. No. orb257612) with an affinity constant of 12.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
38.7 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
38.7 kDa (predicted). Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result. |
> 95% by SDS-PAGE. | |
Recombinant Human IL-5 R alpha/CD125 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Asp21-Glu335) of human IL-5 R alpha/CD125 (Accession #Q01344) fused with a 6��His Tag at the C-terminus. |