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Catalog Number | orb1146968 |
---|---|
Category | Proteins |
Description | Human IL-5 R alpha, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Trp 342 (Accession # Q01344-1). |
Reactivity | Human |
Tag | N-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 63.2 kDa |
Target | IL-5 R alpha, CD125 |
UniProt ID | Q01344 |
Source | Human IL-5 R alpha, Fc Tag (orb1146968) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Trp 342 (Accession # Q01344-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-5, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 0.6-10 ng/mL (QC tested). |
Expression Region | Asp 21 - Trp 342 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000555.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL5Ra,CD125 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 63.2 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IL-5, His Tag (Cat. No. orb612148) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag (Cat. No. orb1146968) with a linear range of 0.6-10 ng/mL (QC tested).
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-hFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |