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Human IL-5 Protein, His Tag
Catalog Number: orb612148
| Catalog Number | orb612148 |
|---|---|
| Category | Proteins |
| Description | Human IL-5, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1). |
| Reactivity | Human |
| Tag | N-10×His |
| Form/Appearance | Powder |
| Purity | 90% |
| Conjugation | Unconjugated |
| MW | 15.0 kDa |
| Target | IL-5 |
| UniProt ID | P05113 |
| Source | Human IL-5, His Tag, premium grade (orb612148) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Ser 134 (Accession # P05113-1). |
| Expression System | HEK293 |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-5, His Tag, premium gradeon NTA Biosensor, can bind Human IL-5 R alpha, Fc Tag with an affinity constant of 4.63 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
| Expression Region | Ile 20 - Ser 134 |
| Endotoxins | 0.1 EU per μg |
| NCBI | NP_000870.1 |
| Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
| Buffer/Preservatives | PBS, pH7.4 |
| Alternative names | IL-5,TRF,IL5,Interleukin-5 Read more... |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 kDa. The protein migrates as 18-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
Loaded Human IL-5, His Tag, premium grade (Cat. No. orb612148) on NTA Biosensor, can bind Human IL-5 R alpha, Fc Tag with an affinity constant of 4.63 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Item 1 of 4
Human IL5 Protein, His Tag [orb689428]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg - Item 1 of 2
Human IL-5 R alpha Protein, His Tag [orb257612]
Unconjugated
85%
37.7 kDa
Human IL-5 R alpha, His Tag (orb257612) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Glu 335 (Accession # NP_000555).
1 mg, 50 μg - Item 1 of 1
Rabbit IL-5 Protein, His Tag [orb750340]
Unconjugated
90%
15.2 kDa
Rabbit IL-5, His Tag (orb750340) is expressed from human 293 cells (HEK293). It contains AA Met 20 - Ser 134 (Accession # G1SL79-1).
1 mg, 50 μg - Item 1 of 1
Mouse IL-5 Protein, His Tag [orb762422]
Unconjugated
90%
15.0 KDa
Mouse IL-5, His Tag (orb762422) is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1).
50 μg, 1 mg - Item 1 of 2
Human IL5RA Protein, His Tag [orb689433]
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
