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Catalog Number | orb257598 |
---|---|
Category | Proteins |
Description | Human IL-4, premium grade is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1). |
Reactivity | Human |
Tag | Native |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 15.0 kDa |
Target | IL-4 |
UniProt ID | P05112 |
Source | Human IL-4, premium grade (orb257598) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-4, premium grade at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag with a linear range of 0.001-0.031 μg/mL (QC tested). |
Expression Region | His 25 - Ser 153 |
Endotoxins | 0.01 EU per μg |
NCBI | AAH67514 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL4,BCGF1,BSF1 Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 15.0 kDa. The protein migrates as 19 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL4 protein
Immobilized Human IL-4, premium grade (Cat. No. orb257598) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha, Fc Tag (Cat. No. orb545738) with a linear range of 0.001-0.031 μg/mL (QC tested).
Greater than 95% as determined by SDS-PAGE. | |
15.1 kDa | |
E.coli |
Greater than 95% as determined by SDS-PAGE. | |
14.97 kDa | |
Mammalian cell |
Greater than 95% as determined by SDS-PAGE. | |
16 kDa | |
Mammalian cell |
> 98% as determined by SDS-PAGE and HPLC. | |
15.0 kDa | |
E.Coli |
Unconjugated | |
95% | |
24.6 kDa | |
Human IL-4 R alpha, His Tag (orb257626) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1). |