Human IL-35 R Protein, His Tag

• Catalog Number: orb1710809
• Category: Proteins
• Description: Human IL-35 R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 & Lys 24 - Asn 622 (Accession # P40189-1(gp130) & Q99665-1(IL12Rb2)).
• Reactivity: Human
• Tag: C-10×His | Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 73.1 kDa | 71.5 kDa
• Target: IL-35 R, gp130 & IL12Rb2
• UniProt ID: P40189, Q99665
• Source: Human IL-35 R, His Tag (orb1710809) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 | Lys 24 - Asn 622 (Accession # P40189-1(gp130) & Q99665-1(IL12Rb2)).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human IL-35 R, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag with a linear range of 0.002-0.625 μg/mL (QC tested).
• Expression Region: Glu 23 - Glu 619 & Lys 24 - Asn 622
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 73.1 kDa & 71.5 kDa. The protein migrates as 95-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-35 R, His Tag (Cat. No. orb1710809) at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag (Cat. No. orb1184774) with a linear range of 0.002-0.625 μg/mL (QC tested).