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Catalog Number | orb1087548 |
---|---|
Category | Proteins |
Description | Human IL-3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 17.0 kDa |
Target | IL-3 |
UniProt ID | P08700 |
Source | Human IL-3 Protein, His Tag, premium grade (orb1087548) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IL-3 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
Expression Region | Ala 20 - Phe 152 |
Endotoxins | 0.3 EU per μg |
NCBI | NP_000579.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL3,MCGF,MGC79398,MGC79399,MULTI-CSF,Interleukin-3 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Human IL-3 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. orb1087548) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Unconjugated | |
90% | |
17.6 kDa | |
Mouse IL-3 Protein, His Tag (orb1101195) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Cys 166 (Accession # P01586-1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 80-90 kDa after removal of the signal peptide. | |
Mammalian |
98.00% | |
16.9 kDa (predicted) same as Tris-Bis PAGE result. |
98.00% | |
16.9 kDa (predicted) same as Tris-Bis PAGE result. |
98.60% | |
16.5 kDa (predicted) |