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Catalog Number | orb257609 |
---|---|
Category | Proteins |
Description | Human IL-27 Ra, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 78.7 kDa |
Target | IL-27 Ra |
UniProt ID | Q6UWB1 |
Source | Human IL-27 Ra, Fc Tag (orb257609) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human IL-27 Ra Protein, Fc Tagimmobilized on CM5 Chip can bind Human IL-27 Protein, Fc Tag with an affinity constant of 3.67 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Gln 33 - Arg 514 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH28003 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 |
Alternative names | IL27RA,CRL1,TCCR,WSX1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 78.7 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-27 Ra protein
Human IL-27 Ra Protein, Fc Tag (Cat. No. orb257609) immobilized on CM5 Chip can bind Human IL-27 Protein, Fc Tag with an affinity constant of 3.67 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Unconjugated | |
90% | |
54.2 kDa | |
Human IL-27 Ra Protein, His Tag (orb1786179) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003.1). |
> 95% by Tris-Bis PAGE | |
Recombinant Human IL-27 Ra / TCCR Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gly34-Lys516) of Human IL-27 Ra / TCCR fused with a hFc His Tag tag at the C-terminal. |