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Catalog Number | orb570284 |
---|---|
Category | Proteins |
Description | Human IL-23 R, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 64.5 kDa |
Target | IL-23R |
UniProt ID | Q5VWK5 |
Source | Human IL-23 R, Fc Tag (orb570284) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Captured Human IL-23 R, Fc Tag on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL23A & IL12B, His Tag(Routinely tested). |
Expression Region | Gly 24 - Gly 355 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_653302.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL-23 R,IL-23 Receptor Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.5 kDa. The protein migrates as 90-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Captured Human IL-23 R, Fc Tag (Cat. No. orb570284) on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL23A & IL12B, His Tag with an affinity constant of 5.36 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Unconjugated | |
90% | |
66.8 kDa | |
Mouse IL-23R, Fc Tag (orb383506) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Asp 372 (Accession # Q8K4B4-1). |
Unconjugated | |
90% | |
39.9 kDa | |
Human IL-23 R, His Tag (orb570286) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1). |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2030, Recombinant Human IL-23 R Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gly24-Gly355) of Human IL-23 R fused with mFc His Tag at the C-terminal. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result. kDa |
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