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Catalog Number | orb257259 |
---|---|
Category | Proteins |
Description | Human IL-2 R beta Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 25.4 kDa |
Target | IL-2 R beta |
UniProt ID | P14784 |
Source | Human IL-2 R beta Protein, His Tag (orb257259) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human IL-2 R beta Protein, His Tag captured on CM5 chip via anti-His antibody, can bind Human IL-2, premium grade with an affinity constant of 1.62 μM as determined in a SPR assay (Biacore T200) (QC tested). |
Expression Region | Ala 27 - Asp 239 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000869.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL2RB,RP5-1170K4.6,CD122,P70-75 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.4 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-2 R beta protein
Human IL-2 R beta Protein, His Tag (Cat. No. orb257259) captured on CM5 chip via anti-His antibody, can bind Human IL-2, premium grade with an affinity constant of 1.62 μM as determined in a SPR assay (Biacore T200) (QC tested).
Unconjugated | |
95% | |
51.0 kDa | |
Human IL-2 R beta, Fc Tag (orb257617) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |