Human IL-2 R alpha / CD25 Protein

• Catalog Number: orb257613
• Category: Proteins
• Description: Human IL-2 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 23.6 kDa
• Target: IL-2 R alpha
• UniProt ID: P01589
• Source: Human IL-2 R alpha, His Tag (orb257613) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Human IL-2 R alpha, His Tag captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Glu 22 - Cys 213
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000408
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL2RA,CD25,p55,IL2-RA,IL-2-RA
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-2 R alpha protein

Human IL-2 R alpha, His Tag (Cat. No. orb257613) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).