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Catalog Number | orb613659 |
---|---|
Category | Proteins |
Description | Human IL-2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 17.3 kDa |
Target | IL-2 |
UniProt ID | P60568 |
Source | Human IL-2, His Tag (orb613659) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,&Fc, on SA Biosensor, can bind Human IL-2, His Tag with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Ala 21 - Thr 153 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000577.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL2,TCGF,lymphokine,Interleukin 2 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.3 kDa. The protein migrates as 18 kDa and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Loaded Biotinylated Human IL-2RB & IL-2RA & IL-2RG, Fc, Avitag & Fc, Avitag on SA Biosensor, can bind Human IL-2, His Tag (Cat. No. orb613659) with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
> 97% as determined by SDS-PAGE and HPLC. | |
15.4 kDa | |
E.Coli |
Unconjugated | |
95% | |
23.6 kDa | |
Human IL-2 R alpha, His Tag (orb257613) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408). |
Unconjugated | |
95% | |
48.4 kDa | |
Human IL-2 R alpha Protein, Fc Tag (orb257608) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408). |
Unconjugated | |
90% | |
25.4 kDa | |
Human IL-2 R beta Protein, His Tag (orb257259) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |
Unconjugated | |
95% | |
51.0 kDa | |
Human IL-2 R beta, Fc Tag (orb257617) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1). |
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