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Catalog Number | orb257583 |
---|---|
Category | Proteins |
Description | Human IL-1RL1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # NP_003847). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 61.0 kDa |
Target | IL-1RL1 |
UniProt ID | Q01638 |
Source | Human IL-1RL1, Fc Tag (orb257583) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # NP_003847). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Loaded Human IL-1RL1, Fc Tag on Protein A Biosensor, can bind with Biotinylated Human IL-33, His,an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Lys 19 - Pro 323 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_003847 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-1RL1 protein
Loaded Human IL-1RL1, Fc Tag (Cat. No. orb257583) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His, Avitag an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
95% | |
36.9 kDa | |
Human IL-1RL1, His Tag (orb257582) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Phe 328 (Accession # Q01638-2). |
Biotin | |
95% | |
38.0 kDa | |
Biotinylated Human IL-1RL1, His, (orb1176547) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # Q01638-1). |
Biotin | |
95% | |
62.5 kDa | |
Biotinylated Human IL-1RL1, Fc, (orb1184775) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # Q01638-1). |